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KMID : 0895820060160030049
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Journal of Oriental Rehabilitation Medicine
2006 Volume.16 No. 3 p.49 ~ p.64
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The Study of Naetakcheongeum-san(Neituoqianjin-san) on Anti-Cancer Effects and Mechanisms in Human Leukemic HL-60 Cells
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Kim Sang-Don
Kwon Kang-Beam
Shin Byung-Cheul
Park Tae-Yong
Park Jong-Ha
Kwon Young-Dal
Song Yung-Sun
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Abstract
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Objectives: The aim of this study was to investigate the apoptotic effects and mechanisms of Naetakcheongeum-san(Neituoqianjin-san) extract (NCSE)-treated on HL-60 leukemic cells.
Methods: It was observed that NCSE induced apoptotic changes in a dose-dependent patterns, which was confirmed by DNA fragmentation and an increase of annexin V+/PI- stained cell population. It was monitored that the activation of caspase-3, -8, -9 enzyme activities and the cleavages of those in NCSE-treated HL-60 cells. We investigated the release of cytochrome c from the mitochondria to the cytosol and the bid cleavage in time- and dose-dependent patterns in HL-60 cells. To clarify the involvement of caspases, we used the inhibitors. Cells treated with the caspase-3 inhibitor, Ac-DEVD-CHO, and the caspase-8 inhibitor, z-IETD-FMK and the caspase-9 inhibitor, z-LEHD-FMK.
Results and conclusions: It is suggested that NCSE-induced apoptosis is predominant through the activation of caspase-3 and -8 in HL-60 cells.
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KEYWORD
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Naetakcheongeum-san(Neituoqianjin-san), HL-60 cell, Apoptosis, Caspases
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